Sunita Shailajan1*, Suman Kumaria2, Suhas Pednekar1, Sasikumar Menon1, Harshvardhan Joshi1, Archana
1Herbal Research Laboratory, Ramnarain Ruia College, Matunga (E), Mumbai 400019, INDIA
2Department of Botany, North-Eastern Hill University, Umshing Mawkynroh, Shillong – 793022, Meghalaya, INDIA.
Background: Flemingia vestita Benth (Fabaceae) is a much-branched trailing herb with tuberous roots, found throughout the Himalayas and Khasi Hills up to an elevation of 8,000 ft. Formononetin, pseudobaptigenin, diadzein and genistein are major isoflavones present in the tuberous root of F. vestita. Materials and Methods: In this research work, tubers of F. vestita were collected from different regions of Northeast India and analyzed for their genistein content using two chromatographic techniques (HPTLC and HPLC). Both the chromatographic methods have been optimized and validated as per ICH guidelines. Results:Comparative evaluation of genistein content in different samples of F. vestita revealed that the marker content varies significantly. Tubers collected from Lyngiong were found to be rich in genistein. Conclusion:Thus, chromatographic characterization of F.vestita using HPTLC and HPLC would enable its rational utilization and these methods can be applied for the quality evaluation of F. vestita.
Keywords: Flemingia vestita Benth, genistein, HPLC, HPTLC.