Khennouf Seddik1*, Benchiekh Dalila1, Djidel Saliha1, Dahamna Saliha1, Amira Smain1,Charef Noureddine2, Baghiani Abderahmane2, Harzallah Daoud3 and Arrar Lekhmici2
1Laboratory of phytotherapy applied to chronic diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, 19000, ALGERIA.
2Laboratory of applied biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, 19000, ALGERIA.
3Laboratory of applied microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, 19000, ALGERIA.
Introduction: Mentha pulegium L. and Matricaria chamomilla L. are used in the Algerian folk medicine. This study was carried out to determine the antioxidant activity of these plants and their polyphenols contents. Methods: The antioxidant capacity of the plant extracts was determined by the scavenging activity against 1,1-diphenyl- 2-picrylhydrazyl (DPPH) and β-carotene/linoleic acid model system. Total phenolic content in these extracts were determined using Folin–Ciocalteu’s reagent. Results: The methanolic extract (MeE) of Mentha pulegium L. showed the highest yield of extraction (14.4%). Whereas, the aqueous extract (AqE) of Matricaria chamomilla L. had the highest yield (18.56%) from total extracted plant material. Moreover, the ethyl acetate extract of Mentha pulegium L. contains high amount of total polyphenols; tannins and flavonoids (191.99 μg gallic acid equivalent/g dry extract; 265.33 μg tannic acid equivalent/g dry extract; 110.37 μg quercetin equivalent/g dry extract; 151.11 μg rutin equivalent/g dry extract) respectively. This extract possessed high antioxidant activity (IC50=0.017 mg/ml) in the DPPH assay whilst the chloroformic extract (ChE) is more efficient in the β carotene/linoleic acid assay with (61.07%). The ChE of M. chamomilla contains the higher value of flavonoids (197.43 μg quercetin equivalent/g) and total polyphenols were abundant in MeE. The ethyl acetate extract of this plant had the highest antioxidant activity (IC50= 0.0111 mg/ml) in the DPPH assay, while chloroformic extract shows appreciable inhibition value (37.15%) in the β carotene/linoleic acid assay. Conclusion: These results provide useful information about the utilization of these plants as natural antioxidants in food and in folk medicine.
Keywords: Antioxidants, polyphenols, Mentha pulegium, pennyroyal, Matricaria chamomilla, chamomile.