Toxicological evaluation of Moringa oleifera Lam seeds and leaves in Wistar rats


Pharmacognosy Communications,2016,6,2,100-111.
Type:Original Article

Toxicological evaluation of Moringa oleifera Lam seeds and leaves in Wistar rats

Ajayi Temitayo Olayemi1*, Moody Jones Olanrewaju1, Akintayo Christopher Oloruntoba2

1Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan. NIGERIA.

2Department of Physiology, College of Medicine and Health Sciences, AfeBabalola University. Ado-Ekiti, NIGERIA.


Miracle tree (Moringa oleifera) as it is popularly called, has been found useful both medicinally and economically. Its consumption both in the raw and as processed preparations has increased a great deal thus making the fast growing plant a highly valued and cultivated one in the tropics and sub-tropics. There is however, little reference to its toxicity profile and evaluation. Hence, this study evaluated the toxicity profiles of the leaves and seeds of M. oleifera and the corresponding effects on vital organs of Wistar rats using the biochemical, heamatological and histopathological indices. Daily doses of 100, 200, 400 and 1000 mg/kg body weight of crude methanol extracts of M. oleifera leaves and seeds were administered orally to 8 groups of 5 rats per group each for 28 days. A control group of 5 rats was also included in the experiment. Heamatological, biochemical and histopathological indices were evaluated by standard methods. Data were analyzed using one way analysis of variance and statistically significant difference was considered at p<0.05, p<0.01 and p<0.001. Histopathological changes were observed in the heart, liver, lungs, spleen and kidneys of rats treated with the extracts at all doses tested. Some other physical changes like agitation, confusion and disorientation were observed at the highest dose tested (1000 mg/kg) of the seed extract. A significant increase (p<0.05) in neutrophil, white blood cell (WBC) and platelet were observed. However, a significant decrease in aspartate amino transferase (AST), alanine amino transaminase (ALT), alkaline phosphatase, (ALP) was also observed. The results suggest that the leaf and seed extracts of M. oleifera could boost immunity and offer hepatoprotective effects.

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs.

Pharmacognosy Communications [Phcog Commn.] is a new quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the  publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.

The journal aims to cater the latest outstanding developments in the field of pharmacognosy and natural products and drug design covering but not limited to the following topics:

  • Pharmacognosy and pharmacognistic investigations
  • Research based ethnopharmacological evaluations
  • Biological evaluation of crude extracts, essential oils and pure isolates
  • Natural product discovery and evaluation
  • Mechanistic studies
  • Method and technique development and evaluation
  • Isolation, identification and structural elucidation of natural products
  • Synthesis and transformation studies

Distinctions:  The most widely read, cited, and known Pharmacognosy Communications and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month

Aim and Scope | Editorial Board | Indexed & Abstracted | Instruction to Authors | Manuscript Submission & Charges

Subjects Covered : Natural Products, Pharmacognosy, Phytochemistry, Marine Pharmacognosy and Zoo Pharmacognosy
ISSN :2249-0159 (Print) ; 2249-0167 (Online) Frequency : Quarterly Rapid at a time publication (4 issues/year)

Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.