Marker Based Standardization of Himalayan Berry: Myrica esculenta Buch.-Ham ex D. Don


Pharmacognosy Communications,2020,10,4,xx-xx.
Published:October 2020
Type:Original Article

Marker Based Standardization of Himalayan Berry: Myrica esculenta Buch.-Ham ex D. Don

Sunita Shailajan

Ramnarain Ruia Autonomous College, Herbal Research Lab (Industrial Co-ordination Centre), Matunga (E); Mumbai, Maharashtra, INDIA.


Background: Myrica esculenta Buch.-Ham ex D. Don (Syn-M. nagi) commonly known as Himalayan berry/ Kaphal/ Katphal is an economically important medicinal plant with multipurpose uses. The medium sized tree is utilized for its bark, flowers, roots and fruits in Ayurvedic and Unani system of medicine due to its therapeutic potential. The fruits of M. esculenta are sold in local markets as jam, squashes, pickles etc and are also consumed by the local populations. However, the therapeutic properties of the fruits remain largely ignored. Objectives: The aim of this study is to develop standard quality control parameters and standardize the extracts of fruits of M. esculenta in terms of bioactive marker content and give it a recognition of a standardized extract before it becomes part of a herbal formulation. Materials and Methods: Standardization of fruits of M. esculenta has been carried out in terms of its pharmacognostic evaluation - physicochemical and phytochemical analysis. Chromatographic separation was achieved to develop the best resolved HPTLC and HPLC fingerprints as a quality control tool. Gallic acid, a bioactive marker, was quantitated from M. esculenta fruits collected from various regions of Uttarakhand, different morphological parts and from marketed formulation using validated HPTLC and HPLC method. Results: The LOD and LOQ levels were found to be 8 and 10µg/mL for HPTLC and 0.25 and 0.50µg/mL for HPLC respectively with a linear response range of 10-500 µg/mL for HPTLC and 0.50-50µg/mL for HPLC. The (r2) was found to be greater than 0.99 using both techniques. The concentration of phytochemical marker varied in samples collected from different regions of Uttarakhand. Variation in the marker content has been also observed from different morphological parts and in marketed formulation. Conclusion: To attain reproducible quality of herbal products selection and use of authentic plant material is of utmost need. Unique fingerprints can be used as a quality control tool for the use of authentic sample of M. esculenta singularly and as a part of various medicinal formulations. The method developed for the estimation of gallic acid can be applied to matrices containing M. esculenta fruits. The findings of the study will enable sustainable harvest of M. esculenta fruits and its processing as a value-added minor forest produce of Garhwal Himalayas.

Cite This Article

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs.

Pharmacognosy Communications [Phcog Commn.] is a new quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the  publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.

The journal aims to cater the latest outstanding developments in the field of pharmacognosy and natural products and drug design covering but not limited to the following topics:

  • Pharmacognosy and pharmacognistic investigations
  • Research based ethnopharmacological evaluations
  • Biological evaluation of crude extracts, essential oils and pure isolates
  • Natural product discovery and evaluation
  • Mechanistic studies
  • Method and technique development and evaluation
  • Isolation, identification and structural elucidation of natural products
  • Synthesis and transformation studies

Distinctions:  The most widely read, cited, and known Pharmacognosy Communications and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month

Aim and Scope | Editorial Board | Indexed & Abstracted | Instruction to Authors | Manuscript Submission & Charges

Subjects Covered : Natural Products, Pharmacognosy, Phytochemistry, Marine Pharmacognosy and Zoo Pharmacognosy
ISSN :2249-0159 (Print) ; 2249-0167 (Online) Frequency : Quarterly Rapid at a time publication (4 issues/year)

Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.