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    Pharmacognosy Communications
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    Pharmacognosy Communications
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    Research Article

    Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract

    wadmin2By wadmin2April 11, 2014Updated:August 10, 2021No Comments2 Mins Read
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    Chikezie, P.C1 and Uwakwe A.A2
    1Department of Biochemistry, Imo State University, Owerri, Nigeria.
    2Department of Biochemistry, University of Port-Harcourt, Port-Harcourt, Nigeria.

    Pharmacognosy Communications,2014,4,2,20-28.
    DOI:10.5530/pc.2014.2.5
    Published:April 2014
    Type:Research Article

    ABSTRACT

    Background/Objective: The present study sought to investigate erythrocyte glutathione S-transferases (GST), NADH-Methaemoglobin reductase (NADH-MR) and Na+/K+-ATPase activities of hypoglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa as an agent of glycemic control. Materials and Methods: Hyperglycemia was induced by a single intra-peritoneal injection of 0.1 mol/L alloxan monohydrate in phosphate buffer saline (PBS) solution (pH =7.4); dosage = 140 mg/kg. At the end of the experimental time (t = 76 h), erythrocyte GST, NADH-MR and Na+/K+-ATPase activities as well as serum fasting blood sugar (FBS) levels were measured by spectrophotometric methods. Results: Serum FBS levels of control/normal (C/N) rats ranged between 72.93±0.82– 95.12±0.92 mg/dL, whereas experimental rats without glycemic control gave: 249.41±1.03–256.11±1.23 mg/ dL. Hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa exhibited comparative reduced serum levels of FBS alongside with erythrocyte GST, NADH-MR and Na+/K+-ATPase activities. The average relative activities of the three enzymes and corresponding order of enzyme activity in hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa was: NADH-MR = 60.99% > GST = 47.81% > Na+/K+-ATPase = 46.81%. In the same order, relative activities of the three enzymes in rats without glycemic control were: NADHMR = 49.65% > GST = 23.69% > Na+/K+-ATPase = 17.02%. Conclusion: Erythrocyte GST, NADH-MR and Na+/K+-ATPase activities gave insights into the pathophysiology of the diabetic state and served as biomarkers for ascertaining therapeutic control in Type 1 diabetes mellitus.

    Keywords: Glutathione S-transferases, NADH-Methaemoglobin reductase, Na+/K+-ATPase, Allium sativa, hyperglycemia, diabetes mellitus.

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    About Journal
    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
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