Site Overlay

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract

Chikezie, P.C1 and Uwakwe A.A2
1Department of Biochemistry, Imo State University, Owerri, Nigeria.
2Department of Biochemistry, University of Port-Harcourt, Port-Harcourt, Nigeria.

Pharmacognosy Communications,2014,4,2,20-28.
DOI:10.5530/pc.2014.2.5
Published:April 2014
Type:Research Article

ABSTRACT

Background/Objective: The present study sought to investigate erythrocyte glutathione S-transferases (GST), NADH-Methaemoglobin reductase (NADH-MR) and Na+/K+-ATPase activities of hypoglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa as an agent of glycemic control. Materials and Methods: Hyperglycemia was induced by a single intra-peritoneal injection of 0.1 mol/L alloxan monohydrate in phosphate buffer saline (PBS) solution (pH =7.4); dosage = 140 mg/kg. At the end of the experimental time (t = 76 h), erythrocyte GST, NADH-MR and Na+/K+-ATPase activities as well as serum fasting blood sugar (FBS) levels were measured by spectrophotometric methods. Results: Serum FBS levels of control/normal (C/N) rats ranged between 72.93±0.82– 95.12±0.92 mg/dL, whereas experimental rats without glycemic control gave: 249.41±1.03–256.11±1.23 mg/ dL. Hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa exhibited comparative reduced serum levels of FBS alongside with erythrocyte GST, NADH-MR and Na+/K+-ATPase activities. The average relative activities of the three enzymes and corresponding order of enzyme activity in hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa was: NADH-MR = 60.99% > GST = 47.81% > Na+/K+-ATPase = 46.81%. In the same order, relative activities of the three enzymes in rats without glycemic control were: NADHMR = 49.65% > GST = 23.69% > Na+/K+-ATPase = 17.02%. Conclusion: Erythrocyte GST, NADH-MR and Na+/K+-ATPase activities gave insights into the pathophysiology of the diabetic state and served as biomarkers for ascertaining therapeutic control in Type 1 diabetes mellitus.

Keywords: Glutathione S-transferases, NADH-Methaemoglobin reductase, Na+/K+-ATPase, Allium sativa, hyperglycemia, diabetes mellitus.

Copyright © 2021 Pharmacognosy Communications. All Rights Reserved. | Catch Sketch by Catch Themes
Scroll Up