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    Pharmacognosy Communications
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    Pharmacognosy Communications
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    Research Article

    Determination of Cucurbitacin B in Trichosanthes dioica Roxb by HPTLC

    wadmin2By wadmin2April 11, 2014Updated:August 10, 2021No Comments2 Mins Read
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    Joy Ashish Solomon, Aditya Ganeshpurkar*, Nilesh Shrivastava1, Divya Bansal1, Nazneen Dubey
    Drug Discovery Laboratory, Department of Pharmacy, Shri Ram Institute of Technology, Jabalpur, M.P., INDIA.
    1Pharmaceutics Research Laboratory, Department of Pharmacy, Shri Ram Institute of Technology, Jabalpur, M.P., INDIA.

    Pharmacognosy Communications,2014,4,2,8-12.
    DOI:10.5530/pc.2014.2.3
    Published:April2014
    Type:Research Article

    ABSTRACT

    Background: Trichosanthes dioica Roxb, (Cucurbitaceae), is a perennial herb distributed in Asian subcontinent. The pointed gourd is mainly cultivated as a vegetable. The Charaka Samhita prescribes its use in treating jaundice and alcoholism. Ayurvedic literature depicts its use as a diuretic, cardiotoinc, antiulcer and a laxative. Recent research trend on Trichosanthes dioica Roxb. has also demonstrated its anticancer potential. Objective: The aim of the current work was to develop a new, simple, sensitive, precise, and robust HPTLC method to determine the cucurbitacin content in fruit extracts of Trichosanthes dioica Roxb Linn. Materials and Methods: Trichosanthes dioica Roxb was collected from Jabalpur, India. The dried fruit powder of Trichosanthes dioica Roxb. was extracted with methanol in several batches by Soxhlet extraction, and subjected to HPTLC analysis. The method was also assessed for precision, robustness, limit of detection, limit of quantification, accuracy, ruggedness and specificity. Results: The present HPTLC method was used to establish a cucurbitacin calibration curve in the concentration range of 40 – 240 ng/spot. Regression analysis showed a good linear relationship with r2 > 0.99982. The method followed the criteria of precision, robustness, limit of detection, limit of quantification, accuracy, ruggedness and specificity as per ICH guidelines. Conclusion: Statistical analysis of the data demonstrated that our method was selective and reproducible for estimation of cucurbitacin B.

    Keywords: Trichosanthes dioica Roxb., cucurbitacin B, analytical method development, ICH, HPTLC.

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    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
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