Hammoudi Roukia, Hajj Mahammed Mahfoud, Ramdane Farah and Ouldelhadj M Didi
University Kasdi Merbah-Ouargla, Biogeochemistry of desert environments Laboratory, Ouargla 30000, Algeria.
This work presents a contribution to the detection of biological activity of some secondary metabolites of Teucrium polium geyrii, (Lamiaceae) from Tamanrasset (southern Algeria). The plant was subjected to two types of phenolic compounds extraction; maceration and Soxhlet apparatus. The concentration of total phenols and flavonoids, was determined by colorimetry. This showed that the ethyl acetate extract obtained by maceration contains the largest amount of total phenols, 89.05±0.50 mg/g equivalent of gallic acid, as well as flavonoids with 0.45±0.001 mg/g equivalent of vitexin. The antibacterial activity was measured on bacterial strains (pathogenic and alterations) Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus cremoris, Clostridium perfringens, Klebsilla pneumoniae, Escherichia coli and Proteus mirabilis, by the Aromatogramme method. These activity determinations showed that the extracts tested are endowed with interesting antibacterial activities, with inhibition zones reaching as much as 3.4±0.3 cm in diameter on Proteus mirabilis.
Keywords: Teucrium polium geyrii, Lamiaceae, medicinal plant, polyphenols, flavonoids, antibacterial activity.