Anil Pareek*a, Sandeep Kumar Yadava, Prashant Deasaia, Ashok Godavarthib and Badri Prakash Nagoria
aLachoo Memorial College of Science & Technology (Autonomous), Pharmacy wing, Jodhpur – 342003 (Rajasthan), INDIA.
bRadiant Research Services Pvt. Ltd., Bangalore – 560 050, INDIA.
Aims: The study aimed to evaluate the antioxidant and hepatoprotective activity of an ethanolic extract from Corchorus depressus (CDEE). Materials and Methods: The total phenolic and flavonoid content of CDEE was determined using standard curve of gallic acid and quercetin respectively. In vitro antioxidant activity was determined by 2,2-diphenyl-1-picryl hydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzo-thiazoline-6-sμlfonic acid) diammonium salt (ABTS) radicals and hydrogen peroxide methods. CDEE was administered orally (200 mg, 400 mg.kg−1.day−1) to rats for 7 days. Silymarin was used as positive control. On the 8th day, the rats were given a single dose of CCl4 (1.0 ml/kg, i.p.) except vehicle control rats. After 24 h of CCl4 administration, plasma markers of hepatic damage, hepatic antioxidants and indices of lipid peroxidation along with microscopic evaluation of liver were assessed in control and treatment groups. Results: The CDEE possessed strong antioxidant activity in vitro. Pre-treatment of CDEE to CCl4 treated rats prevented hepatocyte damage as evidenced by estimating various biochemical parameters and histopathological observations. CDEE significantly prevented CCl4 induced elevation of aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB) and CCl4 induced decrease in total protein (TP) level in Wistar rats. The CDEE treated rat liver anti-oxidant parameters superoxide dismutase (SOD), catalase (CAT), monodialdehyde (MDA) and glutathione (GSH) were significantly antagonized for the pro-oxidant effect of CCl4. Histopathological studies also supported the protective effect of CDEE. Conclusion: This study partially validated the folk and traditional use of snail in liver disorder through CCl4-induced rat experimental model.
Keywords: ABTS, Antioxidant, Corchorus depressus, CCl4, DPPH, Hepatoprotective.