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    Pharmacognosy Communications
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    Pharmacognosy Communications
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    Research Article

    Evaluation of the impact of regional variation on b-sitosterol content in Asteracantha longifolia Nees. seeds using HPTLC and HPLC technique

    wadmin2By wadmin2August 21, 2013Updated:August 10, 2021No Comments2 Mins Read
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    Sunita Shailajan*, Neelam Sayed and Bhavesh Tiwari
    Herbal Research Lab, Ramnarain Ruia College, Matunga (E), Mumbai 400019, INDIA.

    Pharmacognosy Communications,2013,3,3,16-21.
    DOI:10.5530/pc.2013.3.4
    Published:August 2013
    Type:Research Article

    ABSTRACT

    Background: Seeds of Asteracantha longifolia Nees. (Acanthaceae) are an important ingredient in many traditional/herbal formulations used in the management of a wide range of reproductive disorders. They are reported to posses various therapeutically active phytochemical constituents like asteracanthine, lupeol, asterol, β-sitosterol etc. Materials and Methods: In this research work, seeds collected from various geographical regions were analyzed using two chromatographic techniques (HPTLC and HPLC). A comparative evaluation of β-sitosterol content in respective samples using these techniques has been carried out and discussed. Both the chromatographic methods have been validated as per ICH guidelines and applied to evaluate the content of β-sitosterol in formulations containing seeds of A. longifolia. Results: Variation in the β-sitosterol content of A. longifolia seeds collected from different geographical regions was observed. Seeds collected from Aligarh were found rich in β-sitosterol while sample from Pen showed minimum content. The methods were also found applicable to evaluate the β-sitosterol content in some traditional/ herbal formulations of A. longifolia seeds. Conclusions: Impact of geographical variation on the content of β-sitosterol in the plant samples was clearly evident from the findings of the current work. There exists a definite impact of climatic and edaphic conditions over the marker content of medicinal plants.

    Keywords: Asteracantha longifolia Nees., HPLC, HPTLC, regional variation, seeds.

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    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
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