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    Pharmacognosy Communications
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    Pharmacognosy Communications
    retyeyutreu
    Original Article

    In vitro evaluation of anti-inflammatory and antioxidant effects of Asparagus aphyllus L., Crataegus azarolus L., and Ephedra alata Decne.in monocultures and co-cultures of HepG2 and THP-1-derived macrophages

    wadmin2By wadmin2October 6, 2016Updated:August 12, 2021No Comments2 Mins Read
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    Abdalsalam Kmail1,2, Badiaa Lyoussi1, Hilal Zaid2, Hamada Imtara1, Bashar Saad2
    1Physiology-Pharmacology, University of Fez, P.O.Box 1796 Fez Atlas, Fez, Morocco.
    2Qasemi Research Center- Al-Qasemi Academic College, and Faculty of Arts and Sciences, Arab American University Jenin, P.O.Box 240, Jenin, Palestine

    Pharmacognosy Communications,2017,7,1,24-33.
    DOI:10.5530/pc.2017.1.4
    Published: October 2016
    Type: Original Article

    ABSTRACT

    Introduction: Asparagus aphyllus L.,Crataegus azarolus L., and Ephedra alata Decne.have been used in traditional Greco-Arab herbal medicine to treat various metabolic and inflammatory diseases. However, to our knowledge, no scientific evidences regarding their anti-inflammatory effects have been published yet.This study aimedto evaluate the antioxidant and anti-inflammatory properties of Asparagus aphyllus L., Crataegus azarolus L., and Ephedra alata Decne.extracts using in vitro mono and co-culture systems. Methods: In the first phase of the current study we measured the total phenol, flavones and flavonol contents as well as the antioxidant activity using the DPPH scavenging assay, ABTS assay, and reducing power of the three plant extracts. In second phase, we used THP-1-derived macrophages as a monoculture and co-culture with hepatic cell line (HepG2) to assess the effects of the three plant extracts on the production of LPSinduced pro-inflammatory (IL-6 and TNF – ) and anti-inflammatory (IL-10) cytokines. Results: All three extracts exhibit relatively high levels of phenolics, flavones and flavonols, which correlate with their antioxidant capacities. All three extracts were found to modulate the secretion levels of TNF – , IL-6 and IL-10 at non-toxic concentrations as measured with MTT and LDH assays. Conclusion: Taken together, our results suggest that the traditional uses of these extracts as anti-inflammatory remedies may be mediated by their significant antioxidant capacity, inhibition of pro-inflammatory cytokines secretion concomitant with enhancement of anti-inflammatory cytokines secretion. 

    Key words: Anti-inflammatory, Antioxidant; Asparagus aphyllus L.; Crataegus azarolus L.;Ephedra alata Decne.
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    About Journal
    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
    Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
    Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online publication is 45 days.
    Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.

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