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    Pharmacognosy Communications
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    Pharmacognosy Communications
    retyeyutreu
    Original Article

    Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil

    wadmin2By wadmin2October 6, 2016Updated:August 12, 2021No Comments2 Mins Read
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    Mohammed Hamed Al-Shahrani1, Maysa Mahfoud2, Riyasdeen Anvarbatcha2, Md Tanwir Athar2, Abdulrahman Al Asmari2*
    1Department of Pediatric Oncology, Prince Sultan Military Medical City, Riyadh, 11159, Kingdom of Saudi Arabia.
    2Research Center, Prince Sultan Military Medical City, Riyadh, 11159, Kingdom of Saudi Arabia.

    Pharmacognosy Communications,2017,7,1,34-40.
    DOI:10.5530/pc.2017.1.5
    Published: October 2016
    Type: Original Article

    ABSTRACT

    Objective: To evaluate the antimicrobial properties of Thymus vulgaris against seven pathogenic fungi strains and its cytotoxic effect on a human breast cancer cell line (MDA-MB-231) Methods: The susceptibility test was carried out in terms of by determining minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) using micro-dilution methods and additional end point by using MTS chromogenic assay. Cytotoxicity activity was assessed using MTT assay, the viability and the IC50 were used to evaluate this test. Results: All tested fungal strains were sensitive to the (Thymus vulgaris essential oil) TEO and this inhibitory effect is dose-dependent. A remarkable (p<0.001) inhibitory effect at concentrations 2.5-10mg/ml for all tested organisms comparable with control was found. The MIC50 values ranged between 0.5 to 5mg/ml. The MFC values varied from 2.5–10 mg/mL and were equal to MIC values for majority of the tested organisms. As far as the antifungal activity is concern, out of the seven strains tested, Candida kefyr exhibited the highest sensitivity with minimal inhibition growth 2.5mg/ml whereas, Aspergillus niger exhibited the lowest inhibition ≥10mg/ml. Thymus vulgaris oil inhibited proliferation of MDA-MD-231 cancer cells in a time- and dose dependent manner. IC50 value of the complex was found to be time dependent. At 24h treatment, the IC50 values ranging between 108-115μg/ml, while at 48h treatment the values were between 71-78μg/ml against cancer cell. Conclusion: Thymus vulgaris essential oil exhibited significant antifungal and cytotoxic effects on living cells. Therefore, it can be used as alternative antifungal natural substances and also play a significant role in discovery of the new drugs.

    KEY WORDS: Antifungal, cytotoxicity, Thymus, MTT assay, MIC.

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    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
    Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
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