Facebook Twitter Instagram
    Trending
    • Editorial
    • Antimicrobial Activity of Acacia disparrima Benth. and Acacia leiocalyx Pedley Leaf Extracts in Combination with Antibiotics against Bacterial Triggers of Selected Autoimmune Diseases
    • Tasmannia lanceolata (Poir.) A.C.Sm. Berry and Leaf Extracts Inhibit Proliferation and Induce Apoptosis in Selected Human Carcinoma Cell Lines
    • Therapeutic Potential of Arctium lappa L. Root Extracts to Inhibit Gastrointestinal Bacterial Pathogens and Treat Gastrointestinal Disease
    • Bioactive Compounds in Aqueous Extract of Pilis, A Forehead Transdermal Herbal Medicine
    • The Janus Corner
    • Medicinal Plant Images
    • Upcoming Events
    Facebook Twitter Instagram
    Pharmacognosy Communications
    • Home
    • About Journal
      • Aim and Scope
      • Editorial Board
      • Indexing Info
      • Contact Us
    • Browse Issues
      • Articles in Press
      • Current Issue
      • Past Issues
    • For Authors
      • Instructions to Authors
      • Article Processing Charges
      • Submit your article
      • Downloads
    Pharmacognosy Communications
    Original Article

    In vitro effect of Citrullus Colocynthis and Acacia Radiana on Phosphate Calcium Crystallization

    July 31, 2015Updated:August 10, 20212 Mins Read

    Beghalia Mohamed1, Aissa Belouatek2, Said Ghalem2 and Hocine Allali3
    1Department of Biology, University Centre of Tissemsilt, Algeria.
    2Department of Chemistry, Laboratory Environnement, Natural Substances Plant and Food Technology (ESNTA), University Centre of Relizane Algeria.
    3LASNABIO, Department of Chemistry, Faculty of Sciences, University Aboubekr Belkaïd BP 119, Tlemcen, Algeria.

    Pharmacognosy Communications,2015,5,4,257-260.
    DOI:10.5530/pc.2015.4.7
    Published:July 2015
    Type:Original Article

    ABSTRACT

    Introduction: In this work, we performed an in vitro crystallization study enabling the specification of kinetic and thermodynamic conditions of formation and growth of crystalline calcic phosphates species by changing the pH. We used inhibitors, which are medicinal plants which prevent, slow down or reduce crystallization phases. Materials and Methods: We chose the classical model for the study of phosphate crystallization without inhibitor and with it, in order to assess the inhibiting capacity of any chemical species used. The precipitation of the solid phase of phosphates from artificial urine at different initial pH values was the object of our investigation. The crystal size development was monitored by polarized microscopy at different time intervals. After crystallization time, the mixture was filtered, the recovered dried precipitates were analysed by FTIR. In the absence of inhibitor, the crystallization of phosphates at pH=8.00, led to the formation of struvite and Amorphous Carbonated Calcium Phosphates (ACCP), after 4 hours. In presence of inhibitor at pH=8.00 at lower concentrations of sage inhibition was partial. Result and Disucssion: The addition of 1 ml of sage to the mixture decreases the size of crystal, after 4 hours the size of crystals stabilized at 20.67 μm. The complete disappearance of brushite crystals was obtained after addition of 10mL of Citrullus Colocynthis, only Pentahydrated octocalcic phosphates (POP) and ACCP were formed. In the presence of Acacia Radiana, the inhibition of struvite growth and aggregation increased. The addition of up to a volume of 20 mL of Acacia Radiana resulted in total inhibition and crystalline transformation of the ACCP into carbapatite (CA). Phosphate compounds encountered in urine can be dangerous and the use of inhibitors to prevent, slow down or reduce crystallization phases might be very helpful. Conclusion: In this investigation, Citrullus Colocynthis and Acacia Radiana proved to be good inhibitors. Their effect increases with solution pH but they are more efficient in less acidic or neutral urine than in alkaline one.

    Keywords: Acacia Radiana, Citrullus Colocynthis, Crystallization, Inhibition, Struvite.

    Download PDF

    Related Posts

    Antimicrobial Activity of Acacia disparrima Benth. and Acacia leiocalyx Pedley Leaf Extracts in Combination with Antibiotics against Bacterial Triggers of Selected Autoimmune Diseases

    July 20, 2023

    Therapeutic Potential of Arctium lappa L. Root Extracts to Inhibit Gastrointestinal Bacterial Pathogens and Treat Gastrointestinal Disease

    July 20, 2023

    Tasmannia lanceolata (Poir.) A.C.Sm. Berry and Leaf Extracts Inhibit Proliferation and Induce Apoptosis in Selected Human Carcinoma Cell Lines

    July 20, 2023
    Recent Posts
    • Editorial
    • Antimicrobial Activity of Acacia disparrima Benth. and Acacia leiocalyx Pedley Leaf Extracts in Combination with Antibiotics against Bacterial Triggers of Selected Autoimmune Diseases
    • Tasmannia lanceolata (Poir.) A.C.Sm. Berry and Leaf Extracts Inhibit Proliferation and Induce Apoptosis in Selected Human Carcinoma Cell Lines
    • Therapeutic Potential of Arctium lappa L. Root Extracts to Inhibit Gastrointestinal Bacterial Pathogens and Treat Gastrointestinal Disease
    • Bioactive Compounds in Aqueous Extract of Pilis, A Forehead Transdermal Herbal Medicine
    • The Janus Corner
    Facebook Twitter Instagram Pinterest
    © 2023 Phcog.net. Designed by EManuscript.

    Type above and press Enter to search. Press Esc to cancel.

    Scroll Up