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Pseudomonas laurentiana sp. nov., an Mn(III)-oxidizing Bacterium Isolated from the St. Lawrence Estuary

Mitchell Henry Wright1,2,3, John George Hanna1, Derek Anthony Pica II1, Bradley MacLean Tebo1
1Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health and Science University, Portland, Oregon, USA.
2Department of Research and Development, First Choice College, Gold Coast, Queensland, AUSTRALIA.
3School of Natural Sciences, Griffith University, Brisbane, Queensland, AUSTRALIA.

Pharmacognosy Communications,2018,8,4,153-157.
DOI: 10.5530/pc.2018.4.32
Published: October 2018
Type: Original Article

ABSTRACT

Introduction: An aerobic, Mn(III)-oxidizing, Gram-negative, motile bacterium (strain GSL-010T) was isolated from deep waters of the St Lawrence Estuary (Canada) and subjected to a polyphasic taxonomic study. Results: Cells were mostly curved, motile rods (1.75 – 2.2 x 0.5 – 0.7 μm) with growth observed at 10 – 37 ºC (optimum at 30 ºC), pH 5 – 10 (optimum at 7.0 – 7.5) and with 0 – 3% (w/v) NaCl (optimum at 0.5%). Phylogenetic analysis based on 16S rRNA gene sequencing revealed strain GSL-010T as a member of the genus Pseudomonas, most closely related to the type strains of Pseudomonas plecoglossicida (98.9%) and Pseudomonas japonica (98.6%). The major cellular fatty acids of strain GSL-010T are a combination of C16:1 ω7c and/or iso-C15:1 2-OH, C16:0 and C18:1 ω7c. The G+C mol content of the chromosomal DNA is 59.7%. The DNA–DNA hybridization values between strain GSL-010T and P. plecoglossicida (32.0%) and P. japonica (34.4%) confirm the assignment of the bacterium to a new species. Conclusion: On the basis of phylogenetic analysis, DNA–DNA hybridization and physiological and biochemical characterization, strain GSL-010T is clearly a unique bacterium and since it was isolated from the waters of the St. Lawrence estuary, the name Pseudomonas laurentiana sp. nov. is proposed. The type strain is GSL-010T (=JCM 32154T =NBRC 113027T =KCTC 62392T).

Key words: Manganese(III), Mn(III)-L, Pseudomonas,

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