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    Pharmacognosy Communications
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    Pharmacognosy Communications
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    Original Article

    Phytochemical Composition and Potential Genotoxic effects of Important Egyptian Medicinal Plants

    wadmin2By wadmin2June 16, 2015Updated:August 10, 2021No Comments2 Mins Read
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    Khaled Rashed1, Roel Anthonissen2, Davie Cappoen2 and Luc Verschaeve2,3
    1Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt.
    2Operational directorate Food, Medicines and Consumer Safety, Toxicology Unit, Scientific Institute of Public Health, Brussels, Belgium.
    3Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.

    Pharmacognosy Communications,2015,5,3,207-216.
    DOI:10.5530/pc.2015.3.6
    Published:June 2015
    Type:Original Article

    ABSTRACT

    Introduction: We performed a phytochemical analysis and investigated the potential genotoxic properties of ten different methanol extracts of plants that are frequently used in Egypt for medicinal purposes. Such studies are important because these plants were never thoroughly investigated with respect to their possible potential sideeffects and risks for humans. Materials and Methods: In this study we used the bacterial Vitotox test and alkaline comet assay in human C3A cells to estimate their genotoxic potential. The Vitotox test is an interesting screening test which correlates very well with the well-known Ames assay but has the advantage of being rapid, sensitive and requiring only small amounts of a test compound. The test was conducted in the presence and absence of a metabolizing S9-enzyme fraction. The comet assay is now a widely used and validated genotoxicity test which can be applied in all DNA-containing cells. In this study it was conducted in human C3A cells which conserved phase I and II biotransformation capabilities. The in vitro NRU assay was used to investigate toxicity and utilized as a dose-finding test. Results: None of the plants have shown genotoxic properties although one of them, Derris rubusta, showed borderline genotoxicity in both tests. This plant also contains alkaloids and coumarins, besides flavonoids, carbohydrates, tannins and triterpenes that were also found in the other plants. Conclusion: So far all 10 investigated medicinal plants appeared not genotoxic but due to borderline effects, Derris robusta deserves further complementary investigations.

    Keywords: Alkaline comet assay, Egyptian medicinal plants, Methanol extracts, Phytochemical analysis, Vitotox test.

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    About Journal
    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
    Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
    Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online publication is 45 days.
    Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.

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