Iurchenko Nataliia Sergeevna*, IlyinaTatyanaVasilevna, Kovaleva Alla Mihaylovna, Toryanik Erica Leonidivna and Kulish Irina Aleksandrovna
Departament of Pharmacognosy, 4 Bluchera st., The National University of Pharmacy, Kharkiv, Ukraine.
Introduction: Sweet Woodruff (Asperula odorata L.) is a perennial herb that is spread throughout the territory of Ukraine. This herb is widely used in folk medicine of Ukraine as a sedative medicine at neurosis, neurasthenia, hysteria, depression, applied locally at allergic rash, and it is also used at metritis and colpitis in homeopathy. Materials and methods: The air-dried herb of Asperula odoratawas degreased with chloroform and then treated with the mixture of ethyl acetate-alcohol (8:2) to obtain a dry extract. Sedative activity was studied in the “open field” test. The protective effect of dry extract against hypoxia-induced lethality in mice was evaluated by experimental model of as phyctic hypoxia. HPLC analysis was used to identify the phenolic compounds in the dry extract. Results: The dry extract at the dose of 100 mg/kg in the “open field” test caused a significant dose-dependent (p>0.05) decreasing of locomotor activity which has evidenced by potent sedative activity. The dry extract at the dose of 100 mg/kg showed a potent antihypoxic activity which has been confirmed by increasing mice lifetime by 34.8 % in conditions of model hypoxia. In the dry extract, 16 phenolic compounds have been identified and quantified. Conclusion: Our data have provided a rational base for the folkloric use of the dry extract from Sweet Woodruff as a sedative and antihypoxic drug.
Keywords: Antihypoxic activity, dry extract, HPLC, Phenolic compounds, Sedative activity, Sweet woodruff.