Facebook Twitter Instagram
    • Home
    • About Journal
      • Aim and Scope
      • Editorial Board
      • Indexing Info
      • Contact Us
    • Browse Issues
      • Articles in Press
      • Current Issue
      • Past Issues
    • For Authors
      • Instructions to Authors
      • Article Processing Charges
      • Submit your article
      • Downloads
    Facebook Twitter Instagram
    Pharmacognosy Communications
    • Home
    • About Journal
      • Aim and Scope
      • Editorial Board
      • Indexing Info
      • Contact Us
    • Browse Issues
      • Articles in Press
      • Current Issue
      • Past Issues
    • For Authors
      • Instructions to Authors
      • Article Processing Charges
      • Submit your article
      • Downloads
    Pharmacognosy Communications
    retyeyutreu
    Original Article

    Standardized Extract of Mangifera Indica L. Leaves as an Antimycobacterial and Immunomodulatory Agent

    wadmin2By wadmin2March 5, 2016Updated:August 11, 2021No Comments2 Mins Read
    Facebook Twitter Pinterest LinkedIn Tumblr WhatsApp Email
    Share
    Facebook Twitter LinkedIn WhatsApp Pinterest Email

    Sunita Shailajan1,*, Sasikumar Menon2, Savita Kulkarni3, Bhavesh Tiwari1
    1Department of Botany, Herbal Research Laboratory, Ramnarain Ruia College, Mumbai, INDIA.
    2Institute for Advanced Training and Research in Interdisciplinary Sciences (TDM Laboratory), Mumbai, INDIA.
    3Radiation Medicine Centre, Bio-Medical Group, Bhabha Atomic Research Centre, Mumbai, INDIA.

    Pharmacognosy Communications,2016,6,3,137-147.
    DOI:10.5530/pc.2016.3.3
    Published: March 2016
    Type: Original Article

    ABSTRACT

    Background: Mangifera indica L.; the largest fruit-tree found in India, is an important component of indigenous medical systems. Mangifera indica L. leaves (MIL) have been reported to possess various biological activities and thus, can be a useful source of substances for drug development. Although the leaves have been traditionally used as antibacterial and immunomodulatory agent, there is a paucity of scientific data in support of their efficacy. The purpose of this study was an in-depth evaluation of antimycobacterial and immunomodulatory activity of a standardized extract of MIL. Material and Methods: The hexane extract of Mangifera indica L. leaves (HEMIL) was prepared and standardized. Chromatographic characterization of HEMIL was done using validated HPLC and GC-MS/MS technique. HEMIL was evaluated for antimycobacterial and immunomodulatory activity by using various in vitro and in vivo assays. Results and Discussion: HEMIL showed lupeol and stigmasterol content of 21.04 ± 0.03 mg/g and 16.99 ± 0.04 mg/g, respectively; and total terpenoids content of 112.55 ± 2.16 mg LE/g. GC-MS/MS characterization of the extract confirmed the presence of lupeol and stigmasterol and revealed five other phytochemical constituents. The safety of HEMIL was established in vitro and in vivo. HEMIL showed concentration-dependent inhibition of MTB as evident in REMA and radiorespirometry. HEMIL was also found efficacious in immunomodulatory evaluations using RAW 264.7 cells, human PBMCs, cyclophosphamide induced myelosuppressed mice and SRBCs stimulated mice. Conclusion: The promising results not only support the traditional claim of MIL as antibacterial and immunomodulatory agent but also provide data on their use in food supplements for immuno-pharmacological use.

    Key words: Mangifera indica L. Leaves, Triterpenoids, Chromatography, Safety, Efficacy.

    Download PDF
    Share. Facebook Twitter Pinterest LinkedIn Tumblr WhatsApp Email
    About Journal
    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
    Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
    Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online publication is 45 days.
    Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.

    © 2025 Pharmacognosy Communications. Maintained by Manuscript TechnoMedia LLP.

    Type above and press Enter to search. Press Esc to cancel.

    Scroll Up