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    Pharmacognosy Communications
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    Pharmacognosy Communications
    retyeyutreu
    Original Article

    Analyzing Bacterial Species from Different Environments Using Direct 16S rRNA Gene Sequencing Methods

    Web AdminBy Web AdminJanuary 23, 2023Updated:January 23, 2023No Comments2 Mins Read
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    Olubunmi Faniyan1, Victor Akpe2, Ian E Cock2,3,*

    1Department of Science Laboratory Technology, Ekiti State University, Ado-Ekiti, NIGERIA.

    2School of Environment and Science, Nathan Campus, Griffith University, Nathan, Brisbane, Queensland, AUSTRALIA.

    3Centre for Planetary Health and Food Security, Nathan Campus, Griffith University, Nathan, Brisbane, Queensland, AUSTRALIA.

    DOI: 10.5530/pc.2023.1.5

    ABSTRACT

    Multiple bacterial species including Escherichia coli, Bacillus spp., Streptococcus spp., Proteus spp., Pseudomonas aeruginosa, Serratia spp., Staphylococcus aureus, Enteroccus faecalis and Klebsiella spp. were collected and isolated from multiple geographical niches of Ado-Ekiti, Ekiti state in Nigeria. 16S ribosomal RNA (rRNA) gene sequencing analysis was applied using two universal primers. The bacterial isolates had similarity scores ≥ 96% when compared with the sequence-based bacterial type isolates in the GenBank of the National Center for Biotechnology Information (NCBI). The similarity score values were subsequently used to identify the bacterial isolates to genus and species levels. Thus, the inter-specific polymorphisms of the universal primer of the16S rRNA gene among different genera were found to be suitable for this study. Comparison of bacterial 16S rRNA gene sequences has emerged as a preferred genetic technique compared to the phenotypic identification of bacteria based on cell morphology, gram’s reaction, colony morphology, catalase test, sporulation tests, gas production from glucose, e.t.c, and its validity in identifying bacterial species from different environments is verified in this study.

    Keywords: Bacterial species identification, 16S rRNA, Universal primers, Similarity score values, Polymorphisms, Gene sequences.

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    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
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