Jignesh G. Shah1, Dr. Bharat G. Patel2, Sandip B. Patel1, Ravindra Patel1
1Department of Pharmacology, Indukaka Ipcowala College of Pharmacy, New Vallabh vidyanagar, Anand, Gujarat, India. 2Institute of Science & Technology for Advanced studies & Research, Vallabh vidyanagar, Anand, Gujarat, India.
Type: Research Article
Objective: To evaluate its anti-urolithic potential, the ethanolic extract of seeds of Hordeum vulgare was tested in an animal model of urolithiasis. Materials and Methods: Urolithiasis was induced by the addition of 3% glycolic acid to the normal diet of Wistar albino rats for a period of 42 days. Group I served as a normal control. Group II served as urolithiasis control. Group III, IV and V were treated with ethanolic extract of Hordeum vulgare (EHV) at 100, 250 and 500 mg/kg respectively. Group VI was treated with Cystone as a standard drug. The effects of EHV on various biochemical parameters were studied in urolithitic rats. Results: Glycolic acid induced hyperoxaluria in urolithiatic rats. And, there were significant elevated urine output, kidney weight loss and some renal injury markers in glycolic acid induced rats. In vivo antioxidant parameters including lipid peroxidation (MDA), superoxide dismutase (SOD) and catalase (CAT) were also determined. oral administration of EHV 100, 250 and 500 inhibited CaOx crystal disposition in renal tubules and protected against associated changes in polyurea and kidney weight loss. EHV significantly maintained the urinary excretion of the calcium, phosphate, uric acid, urea, and oxalate and increased the excretion of citrate as compared to glycolic acid control animals. The increased deposition of stone forming constituents in the kidneys of calculogenic rats were significantly lowered by treatment with EHV. The extract also induced a significant decrease in MDA which increased in urolithiatic control rats. The extract also significantly increased SOD and CAT in urolithiatic rats which were markedly decreased in glycolic acid induced urolithiasis in rats. Conclusion: This study demonstrates the anti-urolithic activity of Hordeum vulgare seeds and rationalizes their medicinal use for the treatment of urolithiasis.
Key words: glycolic acid; Hordeum vulgare; urolithiasis.