Characterization of the, Antimicrobial, Antioxidant Activity of Proteins from Prosopis cineraria Leaves

Introduction: The present study was designed to screen out the different proteins from Prosopis cineraria (Leaves), known as Shammi (Family Fabaceae) and characterize them by IR, NMR and mass spectrometry and evaluate their antimicrobial potential against various microbial species. Methods: Different leaf extracts were treated with different reagent for the presence and absence of various secondary metabolites. Screening of proteins have been done by electrophoresis. Antimicrobial effects of the proteins were explored against Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus tubingensis. The disc diffusion method was used for determination of antimicrobial effects. Analytical analysis included infrared spectroscopy, nuclear magnetic spectroscopy and mass spectrometry. Results: Phytochemical analysis of different extracts showed the presence of different secondary metabolite including alkaloids, glycosides, tannins, proteins, saponin, phenolic compounds etc. Various proteins were detected with different molecular weights of 10, 25, 30, 35, 48, 50, 55 and 70 KDa. The range of zone of inhibition was 1.4±0.01mm to 14.2±0.18 mm. The maximum activity inhibitory activity was found to be due to the 50 KDa protein against Pseudomonas aeruginosa. Analytical analysis revealed the presence of various functional groups in the proteins. Conclusion: The data generated would be useful for the preparation of monograph of the plant.

Keywords:Antimicrobial, antioxidant, Monograph, Phytochemical analysis, Prosopis cineraria, protein