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    Pharmacognosy Communications
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    Pharmacognosy Communications
    retyeyutreu
    Original Article

    Characterization of the, Antimicrobial, Antioxidant Activity of Proteins from Prosopis cineraria Leaves

    wadmin1By wadmin1April 1, 2019Updated:August 10, 2021No Comments2 Mins Read
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    Getmore Chikowe1, Lindiwe Mpala1, Ian Edwin Cock1,2

    1School of Environment and Science, Griffith University, 170 Kessels Rd, Nathan, Brisbane, Queensland, AUSTRALIA.

    2Environmental Futures Research Institute, Griffith University, 170 Kessels Rd, Nathan, Brisbane, Queensland, AUSTRALIA

    Pharmacognosy Communications,2019,9,2,54-58.
    DOI:10.5530/pc.2019.2.12
    Published: April 2019
    Type: Original Article

    ABSTRACT

    Introduction: The present study was designed to screen out the different proteins from Prosopis cineraria (Leaves), known as Shammi (Family Fabaceae) and characterize them by IR, NMR and mass spectrometry and evaluate their antimicrobial potential against various microbial species. Methods: Different leaf extracts were treated with different reagent for the presence and absence of various secondary metabolites. Screening of proteins have been done by electrophoresis. Antimicrobial effects of the proteins were explored against Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus tubingensis. The disc diffusion method was used for determination of antimicrobial effects. Analytical analysis included infrared spectroscopy, nuclear magnetic spectroscopy and mass spectrometry. Results: Phytochemical analysis of different extracts showed the presence of different secondary metabolite including alkaloids, glycosides, tannins, proteins, saponin, phenolic compounds etc. Various proteins were detected with different molecular weights of 10, 25, 30, 35, 48, 50, 55 and 70 KDa. The range of zone of inhibition was 1.4±0.01mm to 14.2±0.18 mm. The maximum activity inhibitory activity was found to be due to the 50 KDa protein against Pseudomonas aeruginosa. Analytical analysis revealed the presence of various functional groups in the proteins. Conclusion: The data generated would be useful for the preparation of monograph of the plant.

    Keywords:Antimicrobial, antioxidant, Monograph, Phytochemical analysis, Prosopis cineraria, protein

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    About Journal
    About Journal

    Pharmacognosy Communications [Phcog Commn.] is a quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.
    Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
    Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online publication is 45 days.
    Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.

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