Corilagin: First Time Isolation from the Whole Plant of Phyllanthus maderaspatensis L.

Abhilash Kochumadhavan, Priyanka Mangal, LM Sharath Kumar, Badugu Madhura Meenakshi, Babu Uddagiri Venkanna, Ganesh Muguli*

R&D Centre, The Himalaya Drug Company, Bengaluru, Karnataka, INDIA.

Pharmacognosy Communications,2019,9,4,135-138.
Published:September 2019
Type:Original Article


Introduction: Corilagin, an ellagitannin (chemical name β-1-O-galloyl-3,6- (R)-hexahydroxydiphenoyl-d-glucose), was previously reported from other species of Phyllanthus except Phyllanthus maderaspatensis L. Therefore, the aim of the study was to isolate corilagin from P. maderaspatensis and report it as chemo-marker. Methods: The whole plant of P. maderaspatensis was subjected to hot extraction and partitioning, followed by repeated column chromatography (silica, Diaion HP-20 and Prep HPLC). The observed LC‑MS/MS and NMR data were compared with reported literature to verify the structure of the isolated molecule as corilagin. Results: The LC-MS/MS chromatogram showed a peak at retention time 22.05 min, with m/z 633 for [M – H]− along with characteristic major fragments at m/z 463 [M – 170 – H]− and m/z 301 [M – 332 – H]−. The NMR results were also in accordance with literature. Conclusion: This is the first report on isolation of corilagin from P. maderaspatensis whole plant for standardization and quality control of the plant.

Key words: 13C NMR, 1H NMR, Corilagin, Ellagitannin, Phyllanthus maderaspatensis.
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